Antibacterial Activity Test Of Ethanol Extract And N-Hexane Fraction, Ethyl Acetate, Moringa Leaf Water (Moringa Oleifera L) Against Streptococcus Mutans ATCC 25175 Bacteria

Wahdah Sri Rahayu; Tatiana Siska Wardani; Tiara Ajeng Listyani

doi:10.55227/ijhet.v4i6.662

Authors

Wahdah Sri Rahayu Universitas Duta Bangsa Surakarta
Tatiana Siska Wardani Universitas Duta Bangsa Surakarta
Tiara Ajeng Listyani Universitas Duta Bangsa Surakarta

DOI:

https://doi.org/10.55227/ijhet.v4i6.662

Keywords:

Antibacterial, Moringa oleifera L leaves, Extract, Fraction, Streptococcus mutans

Abstract

Dental caries remains a major oral health problem globally and in Indonesia, triggered by Streptococcus mutans biofilm. Chemical agents such as chlorhexidine have side effects, encouraging the use of natural alternatives from Moringa oleifera L. leaves. This study aims to evaluate the antibacterial activity of ethanol extracts and n-hexane, ethyl acetate, and water fractions against Streptococcus mutans ATCC 25175, identify the most active fraction, and determine the MIC and MBC. This quantitative study used diffusion and dilution methods. Bacteria: Streptococcus mutans ATCC 25175; samples: ethanol extract of Moringa oleifera L. leaves and n-Hexane, ethyl acetate, and water fractions. Data analysis used SPSS 25 One-Way ANOVA, Tukey (p<0.05). Results showed that the ethyl acetate fraction was the most active with 12.36 mm inhibition at 45%; MIC and MBC were 30% ANOVA significant (p=0.000). The ethyl acetate fraction has strong caries prevention potential, supporting herbal development.

Downloads

Download data is not yet available.

References

Agustina, C., Mujahid, I., & Dhanti, KR (2023). Effect of dimethyl sulfoxide (DMSO) and different preservation temperatures on Methicillin-Resistant Staphylococcus aureus (MRSA) bacteria, 11, 328–336.http://jmm.ikestmp.ac.id/10.52523/maskermedika.v11i2.563.

Andika, NA, Artini, KS, & Wardani, TS (2022). Extract and active fraction of saga leaves (Abrus precatorius L.) against Streptococcus mutans ATCC 25175 bacteria.

Dilusi, EIF, & Royani, S. (2023). Phytochemical screening and antioxidant testing of cayenne pepper (Capsicum frutescens L.) leaf extract.

Erna, Y., & Azizah, N. (2025). Testing the effectiveness of using hot air batan in making bacterial preparations with gram staining, 7(1), 49–56.

Fatwami, EF, & Royani, S. (2023). Phytochemical screening and antioxidant testing of cayenne pepper (Capsicum frutescens L.) leaf extract, 5, 253–260.

Hasibuan, AS, & Edrianto, V. (2021). Socialization of phytochemical screening of ethanol extract of shallot bulbs (Allium cepa L.), 1(1), 80–84.https://doi.org/10.35451/jpk.v1i1.732.

Kartikasari, D., Fahmi, R., Rahman, IR, Ridha, A., Study of Diploma III Pharmacy, P., Pharmacy Yarsi Pontianak, A., Study of Public Health, P., Health Sciences, F., & Muhammadiyah Pontianak, U. (2023). SPF test of kesum leaves (Polygonum minus Huds) in several solvents using UV-Vis spectrophotometry. Journal of the National Pharmaceutical Community, 3(1).

Kusumo, DW, Ningrum, EK, Hayu, C., Makayasa, A., Pharmacy, PS, Health, FI, Lamongan, UM, & Pipaya, B. (2022). Phytochemical screening of secondary metabolite compounds in ethanol extract of papaya flowers (Carica papaya L.), 5(2).

Kumalasari, E., Septia Arini, DRF, & Noor Aisyah. (2023). Determination of total flavonoid content of hexane ethanol extract, water fraction, ethyl acetate fraction from leaves, 9(C), 173–180.

Muhammad Andira Ibnu Shina, Tatiana Siska Wardani, & Kusumaningtyas Siwi Artini. (2024). Antibacterial activity test of extract, water fraction, ethyl acetate fraction, n-hexane fraction of petai cina (Leucaena leucocephala) leaves against Staphylococcus aureus ATCC 25923 bacteria. Drugs: Journal of Pharmaceutical and Health Science Research, 2(6), 1–37.https://doi.org/10.61132/obat.v2i6.785.

Murdiyansah, S., Rasmi Citra Ayu, D., & Mehrta Gdei, I. (2020). Centella asiatica activities towards Staphylococcus aureus and Escherichia coli growth.

Nurmailah, F., Muawanah, UAI, & Widyantara, BA (2024). Effectiveness of pomegranate as an alternative to safranin, 5, 11501–11507.

Ratna Sulistyorini, MK, Muhammad Munawir, DPM, Malisa Nisaul Alim, AA, & MRSAN (2025). Isolation and identification of bacteria in dental plaque of patients at GM Hospital, Muhammadiyah University of Semarang.

Ririn Puspita, NH, & M. (2024). Antibacterial activity test of methanol extract of kaffir lime leaves (Citrus hystrix DC) using UAE (Ultrasound Assisted Extraction) extraction method against Propionibacterium acnes bacteria using disc diffusion, 4(3), 1863–1878.

Sandy, M., Wardani, TS, & Septiarini, AD (2021). Antibacterial activity test of extract, n-hexane fraction, ethyl acetate fraction, water fraction of gotu kola leaves (Centella asiatica (L.) Urb) against Escherichia coli ATCC 25922, 16(2), 1683–1692.

Utari, B., Munawaroh, R., Farmasi, LB, Farmasi, F., Surakarta, UM, & Yani, JA (2023). Antibacterial activity of ethanol extract of basil leaves and stems (Ocimum basilicum) and its active fraction against Staphylococcus epidermidis, 2(1), 118–132 [file