Antibacterial Activity Test Of Extract And Fraction Of N-Hexane, Ethyl Acetate And Water Of Pineapple Peel (Ananas Comosus (L.) Merr.) Against Staphylococcus Epidermidis ATCC 12228 Bacteria

Nadia Nurul Wachida; Tatiana Siska Wardani; Rahmat Hidayat

doi:10.55227/ijhet.v4i6.670

Authors

Nadia Nurul Wachida Universitas Duta Bangsa Surakarta
Tatiana Siska Wardani Universitas Duta Bangsa Surakarta
Rahmat Hidayat Universitas Duta Bangsa Surakarta

DOI:

https://doi.org/10.55227/ijhet.v4i6.670

Keywords:

Antibacterial Activity, Ananas Comosus, Ethyl Acetate Fraction, Minimum Inhibitory Concentration, Staphylococcus Epidermidis

Abstract

Staphylococcus epidermidis is a bacteria that causes mild skin infections accompanied by abscesses, with a high prevalence in Indonesia, requiring natural alternatives due to antibiotic resistance. This study aimed to evaluated antibacterial activity of 96% ethanol extract and n-hexane, ethyl acetate, water fractions of pineapple peel (Ananas comosus L. Merr.) against S. epidermidis ATCC 12228 and to determined MIC/MBC of the most active fraction. This quantitative and experimental study used the disk diffusion and liquid/solid dilution. methods. Population: pineapple peels from Blitar, East Java; sample: 700 g Simplicia powder. The nstruments included rotary evaporator, incubator, autoklaf, laminar air flow; data were analyzed using the Kruskal-Wallis (SPSS 25). Results showed that extract produced inhibition zones ranging from 5.63–11.43 mm, ethyl acetate fraction 3.80–7.55 mm, water 2.35–6.88 mm, n-hexane was inactive. The MIC and MBC values of the extract were 25%; ethyl acetate fraction MIC and MBC were 50%. Extract, ethyl acetate, and water fractions exhibited antibacterial activity aginst S. epidermidis, with extract being the most effective.

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References

Achmad, MN, Masengi, ASR, Posangi, J., Fatimawali, & Mambo, CD (2024). Phytochemical screening and antibacterial activity test of ethanol extract of binahong leaves (Anredera cordifolia (Ten.) Steenis) against Streptococcus mutans and Escherichia coli. Jurnal Bios Logos, 14(3), 1–13.

Adriana, UH, Nofita, & Marcellia, S. (2024). Activity test of combination of ethanol extract of basil leaves (Ocimum × africanum Lour.) and fragrant pandan (Pandanus amaryllifolius Roxb.) as antibacterial against Salmonella typhi. Journal of Medical and Health Sciences, 11(1), 185–196.

Akbar, DS, Sunarwidhi, AL, & Muliasari, H. (2022). Antibacterial activity of extracts and fractions of Sargassum polycystum from Batu Layar Beach, West Nusa Tenggara. Indonesia Natural Research Pharmaceutical Journal, 7(2), 97–107.

Amelia, A., Putri, DR, Fairish, LL, Afriliany, SP, Kamilah, S., & Fikayuniar, L. (2023). Comparison of phytochemical screening results from the tube method, TLC (Thin Layer Chromatography) and determination of extract content in green coffee beans. Wahana Pendidikan Scientific Journal, 9(16), 115–124.

A'yuni, TA, Sela, Anggraeny, RN, & Karomah, N. (2024). Standardization of Jatropha curcas L. leaf extract for specific and non-specific parameters. Mantra Bhakti, 2(1), 1–8.https://doi.org/10.47575/mb.v2i1.627.

Badriyah, L., & Farihah, DA (2022). Analysis of shallot (Allium cepa L.) skin extraction using the maceration method. Jurnal Sintesis, 3(1), 30–37.

Damayanti, S., Novalina, D., & Hadi, WS (2024). The effect of pH on the stability of henna leaves as an alternative counterstain in Gram staining. Journal of Health Analysis, 13(63), 1–7.

Dewi, BA, Wardani, TS, & Nurhayati, N. (2021). Phytochemistry. Pustaka Baru Press.

Dianawati, N., & Manisha, RZ (2023). Differences between ethanol extracts of ginger (Zingiber officinale var. amarum), ginger (Zingiber officinale var. officinarum), and red ginger (Zingiber officinale var. rubrum) in inhibiting Streptococcus mutans bacteria. Bhakta Dental Journal, 1(01), 13–18.

Faidah, N., Febrina, D., Prabandari, R., & Silvia, A. (2024). Antioxidant activity test of water, n-hexane and ethyl acetate fractions of ethanol extract of purple corn seeds (Zea mays var. Cerratina Kulesh). Scientific Journal of Applied Pharmacy & Health, 2(1), 28–43.

Farren, Meutia, R., Ginting, ANB, & Lubis, AA (2025). Potential antibacterial activity between orange peel extract and fraction against diabetic ulcers. Amanah Health Journal, 9(1), 72–81.https://doi.org/10.57214/jka.v9i1.765.

Fikayuniar, L., Kuswanti, A., Rahmawati, ES, Immelia, RP, & Ismayanti, S. (2023). Literature review article: Identification of non-specific parameters of ethanol extract of butterfly pea flower (Clitoria ternatea L.). Wahana Pendidikan Scientific Journal, 9(16), 502-508. https://doi.org/10.5281/zenodo.8248015

Kementrian Kesehatan Republik Indonesia. (2017). Farmakope Herbal Indonesia Edisi II. Kementrian Kesehatan Republik Indonesia.

Severn, M. M., & Horswill, A. R. (2023). Staphylococcus epidermidis and its dual lifestyle in skin health and infection. Nature Reviews Microbiology, 21(2), 97–111.https://doi.org/10.1038/s41579-022-00780-3