Determination Of Sun Protective Factor (SPF) Values In N-Hexane, Ethyl Acetate, And Water Fractions Of Ethanol Extract Of Tamarind Eggplant (Solanum Lasiocarpum Dunal.) Peel With UV-VIS Spectrophotometry Method

Natalia Riska Angelinda; Septian Maulid Wicahyo; Alfina Nurrahman

doi:10.55227/ijhet.v4i6.685

Authors

Natalia Riska Angelinda Duta Bangsa University, Surakarta
Septian Maulid Wicahyo Duta Bangsa University, Surakarta
Alfina Nurrahman Duta Bangsa University, Surakarta

DOI:

https://doi.org/10.55227/ijhet.v4i6.685

Keywords:

Ethyl Acetate Fraction, Phytochemicals, Solanum Lasiocarpum, Spectrophotometry, Sun Protection Factor

Abstract

Excessive UV exposure causes skin damage, so natural sunscreen alternatives are needed. This experimental descriptive study aims to identify and measure the Sun Protection Factor (SPF) value of ethanol extract and n-hexane, ethyl acetate, and water fractions from Solanum lasiocarpum Dunal fruit peel in vitro using UV-Vis spectrophotometry. The population of the entire skin simplicia from Tanjung Hulu, Pontianak; a sample of 500 g of powder (mesh 40) was extracted and fractionated. Instruments include a Thermo Scientific UV-Vis spectrophotometer (290-320 nm), rotary evaporator, TLC plate; SPF was calculated using the Mansur equation and Lambert-Beer validation linear regression. The results showed the highest SPF of ethyl acetate fraction (36.97 at 500 ppm, high category), while the ethanol extract (9.32), n-hexane (6.71), and water (6.76) were low. Phytochemical screening was positive for alkaloids, terpenoids, phenolics, and flavonoids. In conclusion, the ethyl acetate fraction has optimal potential for the development of sunscreen cosmetics.

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References

Achmad, E., et al. (2024). Title of article related to ethanol-free test. Journal name, volume (issue), page.

Aini, H. (2021). Phytochemical screening and antioxidant activity testing of foxtail millet flour-based cookies as functional food [Thesis]. Hasanuddin University.

Anggoro, AB (2022). Comparison of total flavonoid content of ethanol extracts of purple eggplant (Solanum melongena L.) and sour eggplant (Solanum ferox L.). Stifar Repository, 2–12.https://repository.stifar.ac.id/Repository/article/download/362/436

Ardiansyah, AK, & Ramayani, SL (2022). Sunscreen activity test of kitolod (Isotoma longiflora L.) leaf extract and fraction. Cendekia Journal of Pharmacy, 6(2), 301–306.

Arinta, DN (2019). Standardization of specific parameters of Moringa oleifera Lamk. leaf extract in two growing locations [Thesis]. Wahid Hasyim University.

Azwanida, NN (2015). A review on the extraction methods use in medicinal plants, principle, strength and limitations. Medicinal & Aromatic Plants, 4(3), 196–206.

Damogalad, VA, et al. (2013). In vitro testing of SPF values of several plants. Pharmacon Journal, 2(3), 45–52.

Ministry of Health of the Republic of Indonesia. (2008). Indonesian Herbal Pharmacopoeia (1st Edition). Ministry of Health of the Republic of Indonesia.

Elsyana, V., et al. (2019). Toxicity test and screening of red onion (Allium cepa L.) peel extract. Malahayati Pharmaceutical Journal, 2(1).

Emilia, I., Setiawan, AA, Novianti, D., Mutiara, D., & Rangga. (2023). Phytochemical screening of sungkai leaf extract (Peronema canescens Jack.) by infundation and maceration. Jurnal Indobiosains, 5(2), 95–102.

Fadila, R., & Rahmawati, S. (2023). Evaluation of UV-Vis spectrophotometry capability in determining the absorbance of plant extracts at UV wavelengths. Indonesian Journal of Pharmacy and Science, 15(2), 89–97.

Forestryana Dyera, A. (2020). Phytochemical Screenings and Thin Layer Chromatography Analysis of Ethanol Extract of Jeruju Leaves (Hydrolea Spinosa L.). Scientific and Maritime Pharmacology, 113–124

Harborne, J. B. (1998). Phytochemical methods: A guide to modern techniques of plant analysis. Chapman & Hall.

Hanifa, NI, Wirasisya, DG, Muliani, AE, Utami, SB, & Sunarwidhi, AL (2021). Phytochemical screening of decoction and ethanolic extract of Amomum dealbatum Roxb. leaves. Journal of Tropical Biology, 21(2), 510–518.

Kothari, C.R. (2004). Research methodology: Methods and techniques (2nd ed.). New Age International.

KA, Syarifuddin, Yusriyani, & Dewi, A. (2022). Analysis of total flavonoid content of ethanol extract of tempuyung (Sonchus arvensis) using UV-Vis spectrophotometry method. Fito Medicine: Journal of Pharmacy and Sciences, 12(2), 69–76.

Lisnawati, N., Fathan, NU, & Nurlitasari, D. (2019). Determination of the SPF value of ethyl acetate extract of gedong mango leaves using UV-Vis spectrophotometry. Indonesian Journal of Pharmaceutical Research, 1(2), 157–166.

Mansur, J.S., Breder, M.N.R., Mansur, M.C.A., & Azulay, R.D. (1986). Determinação do factor de proteção solar por espectrofotometria. Anais Brasileiros de Dermalogia, 61(3), 121–124.

Maslahah, N. (2024). Standards for medicinal plant simplicia as herbal preparation ingredients. BSIP Plantation News, 2(2), 1–4.

Mirunalini, S., & Arulmozhi, V. (2011). Phytochemical screening and antioxidant activity of Solanum torvum. Asian Journal of Pharmaceutical and Clinical Research, 4(3), 25–29.

Peratiwi, SG, Tahara, N., Mustikawati, B., Maisyarah, IT, Indradi, RB, & Barliana, MI (2023). Phytochemical screening and TLC profile of extracts and fractions of manggu leuweung (Garcinia celebica L.) leaves. Indonesian Journal of Biological Pharmacy, 3(1), 10–18.

Ramayani, SL, Nugraheni, DH, & Wicaksono, ARE (2021). Effect of extraction method on phenolic and total flavonoid content (Colocasia esculenta L.). Journal of Pharmacy, 10(1), 11–16.

Rosidah, I., Zainuddin, K., Agustini, OB, & Pudjiastuti, L. (2020). Standardization of 70% ethanol extract of chayote (Sechium edule (Jacq.) Sw.) fruit. Farmasains, 7(1), 13–20.

Sari, WY, Yuliastuti, D., & Hidayati, IG (2021). Phytochemical test and antioxidant activity of ethanolic fraction and lime (Citrus aurantiifolia (Christm.) Swingle) peel cream using DPPH method. PHARMACY: Indonesian Pharmacy Journal, 18(2), 351–360.

Setiawan, B. (2020). Pharmaceutical research methodology. Andi Offset.

Shina, MAI, Wardani, TS, & Artini, KS (2024). Antibacterial activity test of extract, water fraction, ethyl acetate fraction, n-hexane fraction of petai cina (Leucaena leucocephala) leaves against Staphylococcus aureus ATCC 25923 bacteria. Obat: Jurnal Penelitian Ilmu Pharmaceutical Dan Kesehatan, 2(6), 1–37.

Skoog, D. A., Holler, F. J., & Crouch, S. R. (2013). Principles of instrumental analysis (7th ed.). Cengage Learning.

Sugiyono. (2019). Quantitative, qualitative, and R&D research methods. Alfabeta.

Tivani, I., Amananti, W., & Rima Putri, A. (2021). Antibacterial activity test of handwash extract of turi leaves (Sesbania grandiflora L.) againstdap Staphylococcus aureus. Manutung Scientific Journal, 7(1), 86–91.

Yohanes, AIF (2022). Determination of sun protection factor (SPF) value and antioxidant activity of 70% and 96% ethanol extract of Chinese new herb (Artemisia vulgaris L.) in vitro [Thesis]. University of Jambi.