Antioxidant Activity And Tirosinase Inhibition Extract Of Red Shoot Leaves (Syzygium myrtifolium)
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Abstract
Skin aging is a natural process accelerated by free radical exposure, UV radiation, and environmental pollution, necessitating effective and safe anti-aging agents. This study aimed to evaluate the potential of red shoot leaves ethanol extract (Syzygium myrtifolium) as anti-aging through antioxidant activity and tirosinase enzyme inhibition assays in vitro. Extraction was performed by maceration using 70% ethanol (1:10) for 12 hours, centrifuged, sonicated, and concentrated using a rotary evaporator. Phytochemical skrining identified flavonoids, alkaloids, saponins, tannins, and triterpenoids. DPPH antioxidant assay showed inhibition percentages of 96.17–99.49% at concentrations of 200-800 ppm. Tirosinase inhibition test resulted in 82.54% inhibition. These results demonstrate that red shoot leaves extract has dual anti-aging potential through antioxidant protection and melanogenesis inhibition mechanisms. This study provides scientific basis for developing anti-aging cream formulations based on safe and sustainable local natural ingredients
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References
Adli, M. A., Abdul Halim Yap, A. H., Payaban, M., & Mohd Zohdi, R. (2025). GC-MS Analysis, Anti-Tirosinase, and Antioxidant Activities of Syzygium claviflorum Fruits Extract. International Journal of Pharmaceuticals, Nutraceuticals and Cosmetic Science, 8(1), 1–8. https://doi.org/10.24191/ijpnacs.v8i1.01
Ahmad, M. A., Lim, Y. H., Chan, Y. S., Hsu, C.-Y., Wu, T.-Y., & Sit, N. W. (2022). Chemical composition, antioxidant, antimicrobial and antiviral activities of the leaf extracts of Syzygium myrtifolium. Acta Pharmaceutica (Zagreb, Croatia), 72(2), 317–328. https://doi.org/10.2478/acph-2022-0013
Crespi, O., Rosset, F., Pala, V., Sarda, C., Accorinti, M., Quaglino, P., & Ribero, S. (2025). Cosmeceuticals for Anti-Aging: Mechanisms, Clinical Evidence, and Regulatory Insights—A Comprehensive Review. Cosmetics, 12(5), 1–20.
Elmi, A., Zengin, G., Said, M. A., Gil-Ortiz, R., Caprioli, G., Piatti, D., Spina, R., Chtita, S., Ricciutelli, M., Abdoul-Latif, F. M., & Laurain-Mattar, D. (2025). Antioxidant, Enzyme and Molecular Docking Tirosinase Inhibitory Activities of Major Polyphenols in Boscia coriacea Graells, Grewia erythraea (Schweinf.) Chiov., Ochradenus baccatus Delile, and Orthosiphon pallidus Royle Ex Benth. Chemistry & Biodiversity, 22(4), e202402498.
Furi, M., Ainun Alfatma, Rahma Dona, Armon Fernando, Fina Aryani, Rahayu Utami, Septi Muharni, Husnawati, Wira Noviana Suhery, & Melzi Octaviani. (2022). UJI INHIBITOR ENZIM TIROSINASE EKSTRAK DAN FRAKSI DAUN KEDABU (Sonneratia ovata Backer) SECARA IN-VITRO. Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan, 8(2), 201–214.
Hanum, T. I. (2018). Formulasi dan Uji Aktivitas Krim Ekstrak Beras Merah (Oryza Nivara L. ) Sebagai Antiaging. Talenta Conference Series: Tropical Medicine (TM), 1(1), 237–244.
Hartanti, A. I., Gde, I. D., Permana, M., & Puspawati, G. A. K. D. (2021). Pengaruh Konsentrasi Etanol Pada Metode Ultrasonikasi Terhadap Aktivitas Antioksidan Ekstrak Daun Gonda ( Sphenoclea zeylanica ) Effect Of Ethanol Concentration In Ultrasonication Method On Antioxidant Activity Of Gonda Leaf Extract ( Sphenoclea zeylanica ). 10(2).
Jayantie, D. D., Farida, Y., & Taurhesia, S. (2022). Aktivitas Antioksidan dan Inhibisi Enzim Tirosinase Ekstrak Etanol Buah Gandaria (Bouea macrophylla Griff.) secara In Vitro. Pharmacoscript, 5(1), 1–12.
Kusio-Targońska, K., Kosheva, N., Wojtanowski, K. K., Gaweł-Bęben, K., Beis, D., & Kukula-Koch, W. (2025). Tirosinase Inhibitors Among Flora of Lubelskie Region—Application of Bio-Chromatographic Approach and Zebrafish Model in Bioactivity Skrining of Plant Material. Molecules, 30(9).
Martin, R. S. H., Laconi, E. B., & Jayanegara, A. (2022). Aktivitas Antioksidan Ekstrak Daun Sambiloto (Andrographis Paniculata) dan Ekstrak Kulit Manggis (Garcinia Mangostana) terhadap Aflatoksin B1 pada Jagung. Jurnal Ilmu Nutrisi Dan Teknologi Pakan, 20(1), 30–37.
Omidian, H., Akhzarmehr, A., & Bertol, C. D. (2025). Natural-based antioxidants in cosmeceuticals: Extraction, bioavailability and skin ageing applications. International Journal of Cosmetic Science, September, 1–34.
Puspita, P. J., Liliyani, N. P. P., & Ambarsari, L. (2022). In silico analysis of active compounds of avocado fruit (Persea americana Mill.) as tirosinase enzyme inhibitors. Current Biochemistry, 9(2), 73–87.
Rahmah, R. et al. (2023) “Phytochemical screening and antioxidant activity testing of avocado leaf ethanol extract (Persea americana Mill.) using the DPPH methodNo Title,” JOURNAL OF PHARMACEUTICAL AND SCIENCES, (1), pp. 9–25.
Sugihartini, A., & Maryati, M. (2022). UJI AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN PUCUK MERAH (Syzygium myrtifolium) DAN PENETAPAN KADAR FENOL TOTAL. Usadha Journal of Pharmacy, 1(3), 267–277.
Sutisna, S. F. Z., & Chaerunisaa, A. Y. (2022). Review: formulasi dan karakterisari fitosom-ekstak hidrofilik & hidrofobik: metode hidrasi lapis tipis dan penguapan pelarut. Medical Sains : Jurnal Ilmiah Kefarmasian, 7(2), 381–394.
Wenas, D. M., Anggun Meilani, P. A., & Herdini. (2022). Uji Antioksidan Infusa D aun berwarna Mer ah dan Hijau dari Pucuk Mer ah (Syzygium m yrtifolium Walp.) dengan Metode DPPH. Jurnal Farmasi Dan Herbal, 5(1), 26–35.